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The goal of the Horváth
Laboratory of Bioseparation Science is to expand the scope
of novel innovative and high performance bioanalytical techniques
in the fields of medicine and life sciences, using high performance
single- and multi-dimensional separation methods, such as
liquid chromatography (HPLC), capillary electrophoresis (CE),
capillary electrochromatography (CEC) and microfabricated
devices preferably connected to mass spectroscopy (MS) and
NMR. The requirement is engendered by the recent need to study
global changes in expression of nucleic acids (genomics),
proteins (proteomics) and the regulation of the biosynthesis
of biologically important small molecules (metabolomics).
One of the main focuses of our research program is the establishment
of a solid theoretical understanding of the physico-chemical
underpinning of the differential migration processes that
are involved in micro-channel based bioanalytical techniques
of great potential, like microbore-liquid chromatography,
capillary electrophoresis, capillary electrochromatography
and microfabricated separation devices. Our fundamental studies
on the theory and applications are expected to support the
design, preparation and use of new column technologies and
stationary phases we plan to tailor make in the Laboratory.
The results of our theoretical studies together with the expertise
of the principal investigators in chromatography, column engineering,
capillary electrophoresis and microfabricated device technologies
will be utilized in embarking on recent bioanalytical challenges,
and upon success it is expected to have a great impact on
the way in which various diseases will be diagnosed and treated.
The advent of microfluidic-based rapid analytical techniques
opens up new horizons in the biomedical field as capable of
revealing global changes in gene expression at the transcriptome,
proteome and metabolome level. Our endeavor will be supported
by faculty members in collaborative institutions and industrial
partners.
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projects
Theoretical
and Practical Aspects of Capillary Electrochromatography
